human adam 17  (R&D Systems)

Journal: Arthritis Research & Therapy

Article Title: ADAM-17 is expressed on rheumatoid arthritis fibroblast-like synoviocytes and regulates proinflammatory mediator expression and monocyte adhesion

doi: 10.1186/s13075-018-1657-1

Figure Lengend Snippet: Expression of ADAM-17 in RA serum and SFs. a ADAM-17 is higher in RA serum than OA and NL serum. b ADAM-17 expression is also higher in RA SFs than OA SFs. c The level of ADAM-17 in RA serum is correlated with disease activity. (n = number of patients). ADAM a disintegrin and metalloprotease family protein, DAS28 disease activity score 28, ESR erythrocyte sedimentation rate, NL normal, OA osteoarthritis, RA rheumatoid arthritis

Article Snippet: Next day, RA, NL serum or recombinant ADAM-17, which was used as a standard biotinylated anti-human ADAM-17 antibody (R&D Systems) was added as a detection antibody, followed by streptavidin-HRP (BD Biosciences).

Techniques: Expressing, Activity Assay, Sedimentation

Journal: Arthritis Research & Therapy

Article Title: ADAM-17 is expressed on rheumatoid arthritis fibroblast-like synoviocytes and regulates proinflammatory mediator expression and monocyte adhesion

doi: 10.1186/s13075-018-1657-1

Figure Lengend Snippet: Expression of ADAM-17 in RA STs and FLSs. a Representative photomicrographs of synovial tissues (STs) from patient with RA. The left panel shows ST staining with rabbit anti-ADAM-17. The middle panel shows staining with mouse anti-collagen-1. The right panel shows merging of the left panel and middle panel . The yellow color indicates ADAM-17 associated with ST synovium. b Representative photomicrographs of ST samples from patient with OA. c and d Representative photomicrographs of RA fibroblast-like synoviocytes (FLSs). Cryosections and culture cells were stained for ADAM-17 ( c ) or control IgG ( d ). (Original magnification ×200 ( a and b ) ×400 ( c and d )) e ADAM-17 mRNA in TNF-α stimulated RA FLSs is increased 2.4-fold compared with nonstimulated RA FLSs. ADAM a disintegrin and metalloprotease family, FLSs fibroblast-like synoviocytes, OA osteoarthritis, RA rheumatoid arthritis

Article Snippet: Next day, RA, NL serum or recombinant ADAM-17, which was used as a standard biotinylated anti-human ADAM-17 antibody (R&D Systems) was added as a detection antibody, followed by streptavidin-HRP (BD Biosciences).

Techniques: Expressing, Staining

Journal: Arthritis Research & Therapy

Article Title: ADAM-17 is expressed on rheumatoid arthritis fibroblast-like synoviocytes and regulates proinflammatory mediator expression and monocyte adhesion

doi: 10.1186/s13075-018-1657-1

Figure Lengend Snippet: The expression of proinflammatory mediators blocking ADAM-17 in RA FLSs. a ADAM-17 siRNA inhibited ADAM-17 expression in RA FLSs. b The expression of fractalkine/CX3CL1 was significantly decreased in TNF-α-stimulated ADAM-17 siRNA-transfected RA FLS-conditioned medium compared with TNF-α-stimulated control siRNA-transfected RA FLS-conditioned medium. (n = number of replicates). ADAM a disintegrin and metalloprotease family protein, FLSs fibroblast-like synoviocytes, siRNA small interfering RNA

Article Snippet: Next day, RA, NL serum or recombinant ADAM-17, which was used as a standard biotinylated anti-human ADAM-17 antibody (R&D Systems) was added as a detection antibody, followed by streptavidin-HRP (BD Biosciences).

Techniques: Expressing, Blocking Assay, Transfection, Small Interfering RNA

Journal: Arthritis Research & Therapy

Article Title: ADAM-17 is expressed on rheumatoid arthritis fibroblast-like synoviocytes and regulates proinflammatory mediator expression and monocyte adhesion

doi: 10.1186/s13075-018-1657-1

Figure Lengend Snippet: Blocking ADAM-17 in RA FLSs reduces THP-1 adhesion. a THP-1 adhesion to ADAM-17 siRNA-transfected RA FLSs was significantly decreased compared with that to control siRNA-transfected RA FLSs. b ICAM-1 on TNF-α-stimulated ADAM-17 siRNA-transfected RA FLSs was significantly decreased compared with that on control siRNA-transfected RA FLSs. (n = number of replicates). ADAM a disintegrin and metalloprotease family protein, RA rheumatoid arthritis, siRNA small interfering RNA

Article Snippet: Next day, RA, NL serum or recombinant ADAM-17, which was used as a standard biotinylated anti-human ADAM-17 antibody (R&D Systems) was added as a detection antibody, followed by streptavidin-HRP (BD Biosciences).

Techniques: Blocking Assay, Transfection, Small Interfering RNA